HPLC vs. LC-MS/MS

What's the Difference?

HPLC (High-Performance Liquid Chromatography) and LC-MS/MS (Liquid Chromatography-Mass Spectrometry) are both analytical techniques used in the field of chemistry. HPLC is primarily used for separating, identifying, and quantifying components in a mixture, based on their interactions with a stationary phase and a mobile phase. It is a versatile technique that can be used for a wide range of applications. On the other hand, LC-MS/MS combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It not only provides information about the identity and quantity of components in a mixture but also offers structural information about the molecules present. LC-MS/MS is particularly useful for analyzing complex samples, such as biological fluids, as it can provide highly sensitive and selective measurements. Overall, while HPLC is a powerful separation technique, LC-MS/MS offers the added advantage of molecular identification and structural elucidation.

Comparison

Further Detail

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